Title:	Metagenomic assembly of nanopore data from Anaeramoeba sp. OCE22C
DNr:	NAISS 2025/22-890
Project Type:	NAISS Small Compute
Principal Investigator:	Jon Jerlström Hultqvist <jon.jerlstrom.hultqvist@icm.uu.se>
Affiliation:	Uppsala universitet
Duration:	2025-08-01 – 2025-11-01
Classification:	10615
Keywords:

Abstract

The Anaeramoebae is a newly described phylum of anaerobic protists from the eukaryote supergroup Metamonada (Táborský et al. 2017). Anaeramoebids are predominantly amoeboid with a flamellian amoeba morphotype and contain a large mass of symbionts close to the nucleus. The symbiont mass is tightly intercalated with double-membraned electron-dense organelles, identified as host MROs. The symbiont cells are not in direct contact with the MROs and are invariably bound by a host-derived membrane, a symbiosome. The Anaeramoeba MROs are hydrogenosomes that likely produce H2, acetate, and propionate as end-products (Stairs et al. 2021). The symbionts are sulfate-reducing Deltaproteobacteria that are predicted to use the end products of the host MROs. We have recently shown by that the symbiosome is connected to the outside of the cell (Jerlström-Hultqvist et al. 2024). However, little is known about the symbionts and genomes of other isolates of Anaeramoeba. One recently described Anaeramoeba-isolate, called OCE22 (Pavlatova et al. 2024), is closely related to Anaeramoeba flamelloides but TEM indicates a symbiosome of a different organization and with unknown symbionts. We are interested in determining the genome of the amoeba host and symbionts and comparing it to existing data to understand the diversity of the phylum and what adaptations underlie the different symbiont organizations. The project is in collaboration with the Čepička group at the Charles University in Prague which originally described Anaeramoeba.