Title:	Cryo-EM studies of the Phosphatidylinositol-4-Phosphate 5-Kinase Type 1 Alpha
DNr:	Berzelius-2023-32
Project Type:	LiU Berzelius
Principal Investigator:	Michael Hall <michael.hall@umu.se>
Affiliation:	Umeå universitet
Duration:	2023-02-15 – 2023-09-01
Classification:	10601
Keywords:

Abstract

PiP5k1a is a phosphatidylinositol-4-phosphate 5-kinase that plays a critical role in regulating membrane trafficking and signaling in eukaryotic cells. Dysregulation of PiP5k1a has been implicated in a variety of diseases, including cancer. Recent studies have shown that PiP5k1a is overexpressed in various cancer types and that its expression levels are associated with poor patient prognosis. Additionally, PiP5k1a has been shown to regulate cancer cell migration and invasion, making it a potential target for cancer therapy. Despite its importance, little is known about the structural characteristics of PiP5k1a. This proposal aims to investigate the structure of PiP5k1a using cryo-electron microscopy (cryo-EM), providing a deeper understanding of the protein and its role in cancer and other cellular processes. Ultimately, this research could lead to the development of new therapies that target PiP5k1a and improve cancer treatment outcomes. Cryo-EM data has been collected at the SciLifeLab Cryo-EM facility and Stanford SLAC and will be processed using the PReSTO software stack available on Berzelius.