Identify 20-mers absent from GRCh37/GRCm38 using BLAT
Title: |
Identify 20-mers absent from GRCh37/GRCm38 using BLAT |
DNr: |
SNIC 2017/1-233 |
Project Type: |
SNIC Medium Compute |
Principal Investigator: |
Silvano Garnerone <silvano.garnerone@scilifelab.se> |
Affiliation: |
Karolinska Institutet |
Duration: |
2017-05-22 – 2017-08-01 |
Classification: |
10604 |
Keywords: |
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Abstract
We have a new set of 20-mers to check for homology against GRCh37 and GRCm38. We need them to be 'orthogonal', i.e., with homology lower than 60%, to use them as barcodes in PCR and FISH experiments.