Title:	transaldolase engineering
DNr:	SNIC 2021/5-534
Project Type:	SNIC Medium Compute
Principal Investigator:	Mikael Widersten <mikael.widersten@kemi.uu.se>
Affiliation:	Uppsala universitet
Duration:	2021-11-30 – 2022-12-01
Classification:	10602 10603
Homepage:	https://www.kemi.uu.se/bmc/forskning/biokemi/forskargrupper/enzymologi-och-riktad-evolution-av-proteiner
Keywords:

Abstract

Transaldolase TalB has been extensively mutated with the aim to construct new enzymes able to catalyze asymmetric aldol addition reaction. Identified TalB variants will be analyzed experimentally for catalytic properties and MD simulations will complement these functional studies in the rationalization of structure-activity-dynamics properties. A parent TalB variant and isolated mutants will be studied. An experimentally determined structure of the parent enzyme (from X-ray crystallography) is available and will be used as starting scaffold for in silico mutagenesis of studied variants, and will also form the baseline for the protein structural dynamics in the study.