iFISH probes design
Title: |
iFISH probes design |
DNr: |
SNIC 2020/5-398 |
Project Type: |
SNIC Medium Compute |
Principal Investigator: |
Magda Bienko <magda.bienko@ki.se> |
Affiliation: |
Karolinska Institutet |
Duration: |
2020-07-30 – 2021-08-01 |
Classification: |
10602 |
Homepage: |
http://ifish4u.com/ |
Keywords: |
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Abstract
We are building a collection of 30 and 40 nt oligonucleotides, which will represent an invaluable resource for the design of FISH probes against DNA and RNA targets, both in human (GRCh19 and GRCh38). To this end, the identification of putative off-targets (i.e., a sequence homology search against genome/transcriptome) represents a crucial step of this project, although laborious and computation-intensive.